Evaluation of the potential of the ethyl acetate extract of sucupira in the in vitro control of Agroathelia rolfsii
DOI:
https://doi.org/10.33837/msj.v9i1.1767Keywords:
Phytopathogen, Biocontrol, Plant extract, Pterodon emarginatusAbstract
Sustainable agricultural systems have advanced, promoting the reduction of dependence on pesticides through the responsible management of natural resources. Among the alternatives for the control of phytopathogens, plant extracts have been widely studied due to their antifungal potential. This study aimed to evaluate in vitro the antifungal activity of the fruit extract of Pterodon emarginatus against the fungus Agroathelia rolfsii. The experiment was conducted at the IF Goiano – Campus Posse Laboratory, using isolates of A. rolfsii from the mycotheca of Campus Urutaí. The extract was obtained by macerating 200 g of fruits in 1000 mL of ethyl acetate, followed by natural evaporation of the solvent for 33 days. To enable its application, the extract was previously emulsified in Eumulgin® CO 40 (PEG-40), at a ratio of 1:1 (v/v). After the solidification of the culture medium (20 mL per plate), the emulsified extract was applied on the surface of the Petri dishes, corresponding to the concentrations of 0.25; 0.375; 0.5; 1.0 and 2.0 µL mL⁻¹ (0.025; 0.0375; 0.05; 0.10 and 0.20% v/v), considering the volume of pure extract of 5, 7.5, 10, 20 and 40 µL. The experimental design was completely randomized, with 11 treatments and five replications. Mycelial growth was evaluated by radial measurements on two orthogonal axes, and the averages were calculated for each treatment. From the obtained data, the percentage of mycelial growth inhibition (MGI) and the mycelial growth rate index (MGRI) were determined. The results indicated that the extract of P. emarginatus showed antifungal activity against A. rolfsii, with significant effects at the evaluated concentrations. Treatments containing only ethyl acetate and Eumulgin® CO 40 did not differ statistically from the control, suggesting that the inhibition may be associated with bioactive compounds present in the extract. It is concluded that the extract of P. emarginatus presents potential as an alternative agent in the control of A. rolfsii, contributing to the development of sustainable phytosanitary management strategies.
References
Alcântara, R. Q., Bertholucci, G. P., Paula, G. O., Costa, L. S., Bertholucci, J. P., & Borges, L. L. (2023). Investigação in sílico de substâncias bioativas da espécie Pterodon emarginatus. Revista Brasileira Militar de Ciências, 9(23), e159. URL: https://rbmc.org.br/rbmc/article/view/159
Araújo, J. A. M. (2014). Nanopartículas, óleos essenciais e extratos vegetais no controle in vitro de fungos fitopatogênicos (Dissertação de mestrado). Universidade Federal Rural do Semi-Árido.
Batalini, C., Stocco L. O., Fernandes, R. T. S., Marques Junior, J. (2020). Avaliações fitoquímica, fitotóxica e antifúngica da entrecasca do caule de Pterodon pubescens Benth (sucupira branca. Brazilian Journal of Development, 6(10), 77589–77607.
Bettiol, W., & Morandi, M. A. B. (2006). Controle alternativo de doenças de plantas. In Anais do III Congresso Brasileiro de Defensivos Agrícolas Naturais (III COBRADAN) (pp. 101–116). COBRADAN.
Brito, E. O. S. (2019). Análise fitoquímica e avaliação da atividade antioxidante e antimicrobiana de extratos de folhas de Bauhinia forficata (Trabalho de conclusão de curso) Universidade Federal do Amazonas. URL: https://riu.ufam.edu.br/bitstream/prefix/8343/6/TCC_ElizaBrito.pdf
Donati, M., Mondin, A., Chen, Z., Miranda, F. M., Nascimento, B. B., Schirato, G., Pastore, P., & Froldi, G. (2015). Radical scavenging and antimicrobial activities of Croton zehntneri, Pterodon emarginatus and Schinopsis brasiliensis essential oils and their major constituents. Natural Product Research, 29(10), 939–946. DOI: https://doi.org/10.1080/14786419.2014.964709
Ferreira, C. F., Aoyama, E. M., Souza, A. D., Fortes, N. L. P., & Furlan, M. R. (2020). Óleos essenciais e eugenol no controle in vitro de fungos fitopatogênicos de pós-colheita. Revista Biociências, 26(2), 1–12.
Froldi, G., Benetti, F., Mondin, A., Roverso, M., Pangrazzi, E., Djeujo, F. M., & Pastore, P. (2023). Pterodon emarginatus seed preparations: Antiradical activity, chemical characterization, and in silico ADMET parameters of β-caryophyllene and farnesol. Molecules, 28(22), 7494. DOI: https://doi.org/10.3390/molecules28227494
Hillen, T., Schwan-Estrada, K. R. F., Stangarlin, J. R., & Cruz, M. E. S. (2012). Atividade antimicrobiana de óleos essenciais no controle de fitopatógenos. Revista Brasileira de Plantas Medicinais, 14(3), 439–445. DOI: https://doi.org/10.1590/S1516-05722012000300003
Lemos, J. A., Oliveira, A. E. M. F. M., Araujo, R. S., Townsend, D. M., Ferreira, L. A. M., & Barros, A. L. B. (2021). Recent progress in micro and nano-encapsulation of bioactive derivatives of the Brazilian genus Pterodon. Biomedicine & Pharmacotherapy, 143, 112165. DOI: https://doi.org/10.1016/j.biopha.2021.112165
Martins, W. S., & Muraishi, C. T. (2019). Aspectos biométricos e efeito fungitóxico do óleo essencial de sucupira. Revista Integração Universitária, 13(20), 17–24. DOI: https://doi.org/10.31501/1982-9280.2019V13N20p.17-24
Oliveira, M. M. M., Brugnera, D. F., Cardoso, M. G., Guimarães, L. G. L., & Piccoli, R. H. (2011). Rendimento, composição química e atividade antilisterial de óleos essenciais de espécies de Cymbopogon. Revista Brasileira de Plantas Medicinais, 13(1), 8–16. DOI: https://doi.org/10.1590/S1516-05722011000100002
Pacheco, L. B., & Alves, V. A. (2020). Metabólitos secundários de plantas. Revista Agrotecnologia, 11(1), 54–67. DOI: https://doi.org/10.31668/agrotec.v13i2
R Core Team. (2023). R: A language and environment for statistical computing. R Foundation for Statistical Computing.
Redhead, S. A., & Mullineux, S. T. (2023). Nomenclatural novelties. Index Fungorum, 554, 1. http://www.indexfungorum.org/Publications/Index%20Fungorum%20no.554.pdf
Ribeiro, D. A., Macêdo, D. G., Oliveira, L. G. S., Saraiva, M. E., Oliveira, S. F., Souza, M. M. A., & Menezes, I. R. A. (2014). Potencial terapêutico e uso de plantas medicinais em uma área de Caatinga no estado do Ceará, Nordeste do Brasil. Revista Brasileira de Plantas Medicinais, 16(4), 912–930. DOI: https://doi.org/10.1590/1983-084X/13_059
Sá-Filho, G. F., Silva, A. I. B., Costa, E. M., Nunes, L. E., Ribeiro, L. H. F., Cavalcanti, J. R. L. P., Guzen, F. P., Oliveira, L. C., & Cavalcante, J. S. (2021). Plantas medicinais utilizadas na Caatinga brasileira e o potencial terapêutico dos metabólitos secundários: Uma revisão. Research, Society and Development, 10(13), e21096. DOI: https://doi.org/10.33448/rsd-v10i13.21096
Santos, E. L., Farias, M. T. P., Pinheiro, F. C., Nunes, G. R. P., Barros, E. P. M., Silva, D. M. R., & Silva, J. V. (2024). Impactos causados pelo uso excessivo de agroquímicos na agricultura familiar: uma revisão. Observatório de la Economía Latinoamericana, 22(3), e3746. DOI: https://doi.org/10.55905/oelv22n3-113
Santos, I. P. S. (2010). Alternative control of Sclerotium rolfsii in cowpea (Dissertação de mestrado). Universidade Federal de Alagoas.
Santos, J. G. (2024). Composição química e bioatividades de extratos vegetais de Pterodon pubescens (Dissertação de mestrado). Instituto Federal Goiano.
Venturoso, L. R. (2009). Extratos vegetais no controle de fungos fitopatogênicos à soja (Dissertação de mestrado). Universidade Federal da Grande Dourados.
Xavier, M. N. (2023). Incorporação e bioatividade de óleos essenciais de sálvia e sucupira em filmes biodegradáveis (Tese de doutorado). Instituto Federal Goiano.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Maria Eduarda Moura Oliveira, Alexandre Ribeiro Alves Santos, Ana Beatriz Ferreira Souza, Maria Eduarda Poczwardowski, Ingryd Barbosa Costa, Emiliane dos Santos Belo, Milton Luiz da Paz Lima, Melina Korres Raimundi, Rejane Araújo Guimarães
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish in this journal agree to the following terms:
a) The Authors retain the copyright and grant the journal the right to first publication, with the work simultaneously licensed under the Creative Commons Attribution License that allows the sharing of the work with acknowledgment of authorship and initial publication in this journal.
b) Authors are authorized to assume additional contracts separately, for non-exclusive distribution of the version of the work published in this journal (eg, publishing in institutional repository or as a book chapter), with acknowledgment of authorship and initial publication in this journal.
c) Authors are allowed and encouraged to publish and distribute their work online (eg in institutional repositories or on their personal page) at any point before or during the editorial process, as this can generate productive changes, as well as increase impact and citation of the published work.
